The secondary structure of the 5′-noncoding region of beet necrotic yellow vein virus RNA 3: evidence for a role in viral RNA replication
نویسندگان
چکیده
منابع مشابه
Beet Necrotic Yellow Vein Virus Noncoding RNA Production Depends on a 5′→3′ Xrn Exoribonuclease Activity
The RNA3 species of the beet necrotic yellow vein virus (BNYVV), a multipartite positive-stranded RNA phytovirus, contains the 'core' nucleotide sequence required for its systemic movement in Beta macrocarpa. Within this 'core' sequence resides a conserved "coremin" motif of 20 nucleotides that is absolutely essential for long-distance movement. RNA3 undergoes processing steps to yield a noncod...
متن کاملNucleotide Sequence of Beet Necrotic Yellow Vein Virus RNA-1
The complete nucleotide sequence of beet necrotic yellow vein virus RNA-1 is presented. The R N A molecule is 6746 nucleotides long excluding the poly(A) tail and has one long open reading f lame encoding a polypeptide of M r 237 389. The 3' terminal 60 residues of BNYVV RNA-1 display extensive sequence homology with the corresponding portions of BNYVV RNA-2, -3 and -4. Addi t ional 3' terminal...
متن کاملNucleotide Sequence of Beet Necrotic Yellow Vein Virus RNA-2
The complete nucleotide sequence of beet necrotic yellow vein virus (BNYVV) RNA-2 has been determined from a study of cloned cDNA. The RNA sequence is 4612 nucleotides in length, excluding the poly(A) tail. There are six long open reading frames (ORFs) in the sequence. The viral coat protein cistron is the ORF nearest to the 5' terminus and the coat protein amber termination codon is followed b...
متن کاملNucleo-cytoplasmic shuttling of the beet necrotic yellow vein virus RNA-3-encoded p25 protein.
The protein p25 encoded by beet necrotic yellow vein virus (BNYVV) RNA-3 is involved in symptom expression of infected plants. Confocal microscopy analysis of wild-type and mutated p25 fused to GFP and transiently expressed in BY-2 tobacco suspension cells identified a nuclear localization signal (NLS) in the N-terminal part of the protein. Functionality of the NLS was confirmed by pull-down as...
متن کاملFunctional characterization of the Beet necrotic yellow vein virus RNA-5-encoded p26 protein: evidence for structural pathogenicity determinants.
A Beet necrotic yellow vein virus isolate containing a fifth RNA is present in the Pithiviers area of France. A full-length cDNA clone of RNA-5 was obtained and placed under the control of a T(7)-RNA-pol promoter that allowed the production of infectious transcripts. "Pithiviers" isolate-specific necrotic symptoms were obtained on Chenopodium quinoa when RNA-5-encoded p26 was expressed either f...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1993
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/21.6.1389